Table of Contents

Bioluminescence Imaging: Basics and Practical Limitations.- Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells.- Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter.- Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture.- Split Gaussia Luciferase for Imaging Ligand-Receptor Binding.- Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell.- Bioluminescence Reporter Gene-Based Detection of MicroRNAs.- Monitoring of Transcriptional Dynamics of HIF and NFκB Activities.- Real-Time Bioluminescent Tracking of Cellular Population Dynamics.- Fabrication of Bioluminescent Capsules and Live-Cell Imaging.- The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice.- Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis.- Bioluminescence Imaging of Fungal Biofilm Development in Live Animals.- Bioluminescent Imaging of Bacteria During Mouse Infection.- Cell-Based Bioluminescence Screening Assays.- Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice.- Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase.- Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay.- Non-Invasive In Vivo Monitoring of Extracellular Vesicles.- In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL).

Reviews

From the reviews:“The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. … The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields.” (Omer Iqbal, Doody's Book Reviews, January, 2014)